This activity should be graded in several parts such as
listed below..
SAMPLING 100 pts.
1. _____ Student properly mounted sample container on
sampling pole. 2. _____ Student submerged sample bottle and took
sample in a sterile fashion without taking any extraneous flora or fauna.
3. _____ There was no contamination due to touching
of the lip of the bottle.
4. _____ The bottle was properly closed and stored on ice
for transport to lab.
5. _____ Sample bottle was properly labeled.
Assembling of the laboratory apparatus
100 pts.
1. _____ Student properly assembled filtration flask
and pump. 2. _____ Student properly prepared filter pad. 3. _____
Student properly labeled petri dish. 4. _____
Student checked that incubator was at correct temperature.
5. _____ Student sterilized forceps.
Water sample analysis 100 pts.
1. _____ Student properly filtered sample and
recorded sample volume on data sheet. 2. _____ Student
properly transferred the filter to filter pad. 3. _____
Student incubated petri dish upside down at correct temperature for 18 to 24
hours. 4. _____ Student used microscope
properly to count colonies. 5. _____ Student
recorded colony counts properly on data sheet.
Final mathematical analysis and conclusions
100 pts.
1. _____ Student computed E. Coli concentration
correctly. 2. _____ Student computed total coliform concentration
correctly. 3. _____ Student averaged replicate results
if replicate measurements were made. 4. _____
Student determined if the results exceeded state or federal water quality
criteria. 5. _____ Student filled out all of
the other information on the datasheet.
Note: Further data interpretation including graphing
and computation of percent differences depend on which of the research
themes are selected as an educational activity.
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