Objectives

Standards Addressed Biology I Standards
Physical Science Standards

Introduction and Background

Methods Safety            Sample Collection Sample Preservation & Storage  Bacterial Analysis  Calculations

Classroom Activity
Geographic Sources
Effects of Stormwater
Effects of Temperature
Effects of Sunlight
Effects of Salinity
Effects of Soil             Effects of Time

Resources and Links

Teacher Guide

Glossary

Supplies                         Equipment                              Links

Supplies

                           ITEM                                              SOURCE             CATALOG  NO.         COST/QTY

Millipore Petri Dish with sterile filter pad	Fisher Scientific	PD10-047-S0	$ 24.86 / 100 units
Nalgene Filter Funnel	Fisher Scientific	09-740-30D	$ 107.73 / 50 units
mColiBlue24 Media (for Total Coliforms and E. Coli)	Hach	26084-20	$ 42.08 / 20 units
m-FC with Rosolic Acid (for fecal coliforms)	Hach	24285-20	$16.70 / 20 units
KF-Streptococcus (for Enterococcus)**	Hach	28127-50	$43.00 / 50 units
Hardy Diagnostics Dilu-Lok* Dilution Vials, 99 ml	Fisher Scientific	02-686-202B	$50.14 / 50 units
Sterile Sample Containers	Fisher Scientific	09-730-91A	$39.20 / 100 units
Total Startup Supply Cost for first 40 samples using mColiBlue24 media			$ 323.71
Per Sample Cost on Continuing Basis			$ 5.10

* NOTE:  These costs are an approximation.  For example, state contract prices are lower.  Remember to budget for shipping and taxes.
**Not a standard method.

Equipment

1. Sampling pole.  Wal Mart sells an expandable pole used for cleaning pools for about $25.  

2. Vacuum pump, either hand pump, or mechanical. If using a mechanical pump (Fisher Cat# 01-051-1A, approximately $325), set the vacuum to 25mm Hg.  If using a hand pump (Hach Cat# 14283-00, approximately $57), you must pump for an additional 45 seconds to 1 minute to ensure that all the water has been removed.  This is very important. 

3. Filter forceps that can be sterilized under a flame.

4. Bunsen Burner (and a source of natural gas).

5. Filter Flask, Side-arm Filtering Polypropylene (500mL) (Hach Cat# 202899-49, approximately $ 12.50)

6. Three-way polypropylene stopcock, 4 mm bore (optional) (Fisher Scientific Cat # 14-630-2C, approximately $28.33)

7. Pressure and Vacuum Tubing (optional) (Fisher Scientific Cat #14-173C, approximately $39.02 for 12')

8. Number 7 rubber stopper for filter flask  This stopper needs to have one hole to accommodate the adapter for the filter funnel..  These adapters are included in each box of filter funnels.

9. Vacuum Rubber Tubing to attach the side-arm filter flask to vacuum source.

10. Ring stand and ring to hold filter flask.

11. Ampoule Breaker (Hach Cat# 24846-00, approximately $10.00)

12. Permanent Marker for writing on petri dishes.

13. Incubator (Fisher Cat #11-700-107, approximately $445).  These can also be made using a styrofoam cooler and a light bulb or heating pad for heat.

14. Microscope (10 to 15 X magnification) and light source.

15. Hand Tally Counter (optional) for keeping track of the number of colonies counted (Hach Cat # 14696-00, approximately $12.19)
    

LINKS

South Carolina Department of Health and Environmental Control (this is the link to their Outreach & Education page)

US EPA Office Of Water  

(See Water Watch Program, Volunteer Monitoring Resources - www.epa.gov/volunteer )

Hach, Inc.

Fisher Scientific